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Thyroiditis is characterized by both local and systemic manifestations of inflammation. Recent data suggests that nitric oxide (NO) is important in the pathogenesis of autoimmune thyroiditis in experimental animals. We hypothesized that the inducible form of NO synthase (iNOS) may be involved in the inflammatory process and/or in the destruction of thyroid parenchyma in human autoimmune thyroiditis. Quantitative immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR) techniques were used to determine the presence and quantity of iNOS, as well as other immunoreactive molecules in thyroid glands from autoimmune thyroiditis and normal controls. Immunoreactive iNOS, CD3, CD20 and c-fos mRNAs were present in the thyroid glands from normal controls but not from patients with autoimmune thyroiditis. The mean number of immunostaining cells in the thyroid glands from patients with autoimmune thyroiditis was significantly higher for iNOS, CD3 and c-fos than for healthy control subjects. The mean number of CD20-immunostaining cells was higher in the autoimmune thyroiditis group than in the control group, although it did not reach statistical significance. The mean number of cells that were double immunostained for iNOS and CD3 was significantly increased in the autoimmune thyroiditis group. We conclude that iNOS is
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