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[video2brain] Word 2010 – Les Indispensables .rar
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[video2brain] Word 2010 – Les Indispensables.rar
[video2brain] Word 2010 – Les Indispensables.rar
[video2brain] Word 2010 – Les Indispensables.rar
[video2brain] Word 2010 – Les Indispensables.rar
[video2brain] Word 2010 – Les Indispensables.rar
[video2brain] Word 2010 – Les Indispensables.rar
The in vivo and in vitro apoptotic potential of the combination of TNF-alpha and cycloheximide in a rat model of endotoxemia.
The present study was designed to investigate the effects of combined treatment with TNF-alpha and cycloheximide, a protein synthesis inhibitor, on leucocyte apoptosis induced by TNF-alpha alone in endotoxemic animals. The time-course of leucocyte apoptosis, the vascular endothelial damage in mice and the ability of these cells to bind TNF-alpha in vitro was also analysed. This study was carried out in 12-14-week-old C3H/HeN male mice and in 48 male and female Sprague-Dawley rats. TNF-alpha (5 microg kg(-1)) or 5 mg kg(-1) of cycloheximide was given intravenously at 1 h after LPS challenge (3 mg kg(-1)) or by itself. The mice and the rats were then killed at 6, 12, 24 and 48 h after treatment. The levels of circulating apoptotic leucocytes and vascular permeability, along with the TNF-alpha receptor expression on murine leucocytes, were measured at different times following TNF-alpha administration alone and in combination with cycloheximide. Treatment of endotoxemic mice with TNF-alpha alone produced a significant increase in TNF-alpha binding on leucocytes when compared with control. Nevertheless, the combination of TNF-alpha and cycloheximide reduced leucocyte apoptosis by 64% at 24 h and 62% at 48 h after LPS-induced endotoxemia. In LPS-treated rats, the combination of TNF-alpha and cycloheximide induced significantly less leucocyte apoptosis than TNF-alpha alone at all the times studied. Administration of cycloheximide reduced the number of apoptotic leucocytes as
https://colab.research.google.com/drive/11nfT-6T7GZlJTwm7Bliyv5awWWNNstsn
https://colab.research.google.com/drive/1Pu9d_Cyrq_GUdsMyW79WUW2wkLl0UwBJ
https://colab.research.google.com/drive/1bPta5g6VQjlCF2MS3Z5aG6tE_eaQrILi
https://colab.research.google.com/drive/1XTWPoGZ9p0pPWg7UDL18WRMEWZZ7kaQE
https://colab.research.google.com/drive/1j2J9JL4eMy6H6S7WuvYeyzX8-0ZsDgej
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